樟芝深層培養液抗氧化及抗腫瘤特性之研究 (Chinese Edition) Download Epub Mobi Pdf Fb2

 

 

 

樟芝深層培養液抗氧化及抗腫瘤特性之研究 (Chinese Edition)

by 祖瑩 宋

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本書欲探討樟芝深層培養萃取物的抗氧化及抗腫瘤特性。乃將樟芝深層培養萃取物分為濾液乾燥物(DMF)和不同溶劑之菌絲體萃取物與培養基乾燥物(DMCM)進行抗氧化及自由基清除能力之探討。在不同的測試系統中,DMCM的抗氧化能力均不如DMF,顯示DMF中之主要抗氧化成分應來自於菌絲體的二次代謝產物。可見DMF可能具有開發為預防自由基相關疾病之化學預防療劑的潛力。本文也探討ACSC之發酵濾液乾燥物(DMF)是否具有保護H2O2誘導HepG2產生細胞毒性及SD大鼠產生肝毒性的效果,並探討其具有保護效果的可能機制。研究結果發現,推測DMF具有提升大鼠肝組織的活性以維持肝組織之GSH/GSSH ratio恆定比率,及直接清除CCl4代謝產生的自由基,因此具有預防活體內氧化傷害的潛力。本文亦探討樟芝深層培養菌絲體甲醇萃取物(MEM)之抑制癌細胞存活能力並評估其毒殺作用機制,以期找出可能的調控路徑。研究結果顯示,以不同濃度之MEM處理人類肝癌細胞株,由細胞形態變化、DNA斷鏈分析、細胞週期變化,MEM對肝癌細胞之毒殺路徑是經由誘導雕亡而非壞死作用。最後本文將樟芝深層培養菌絲體甲醇萃取物(MEM)以高效能液相層析分離區分後,以抑制肝癌細胞(Hep3B)存活能力來評估各區分物之抗癌效果,並選擇含量較多及抗癌能力較佳之區分物進行再純化及鑑定。

 

 

Details:
rank:
price: $40.00
bound: 200 pages
publisher: 金琅学术出版社 (April 25, 2017)
lang: Chinese
asin:
isbn: 3330824336, 978-3330824331,
weight: 12.2 ounces (
filesize:

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

樟芝深層培養液抗氧化及抗腫瘤特性之研究 (Chinese Edition) 祖瑩 宋

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